Associate Dean for Research and Professor of Pharmacology, Louisiana State University Health Sciences Center-New Orleans

LSU Superfund Research Program leader - Pollutant-Particle Systems and Xenobiotc Bioactivation

Contact

Phone (504) 568-6557

Fax (504) 568-6888

Email wbacke@lsuhsc.edu

Websites http://www.medschool.lsuhsc.edu/pharmacology/faculty/Backes.htm

http://www.medschool.lsuhsc.edu/pharmacology/faculty_detail.aspx?name=backes_wayne

Research interests, expertise

Organization and Complex Formation among Multiple Cytochrome P450 Enzymes in the Endoplasmic Reticulum, Interaction of Heme Oxygenase, Cytochrome P450 their Electron Transfer Partners in the Endoplasmic Reticulum

Recent publications

Huber III, W.J., Marohnic, C.C., Peters, M., Alam, J., Masters, B.S.S., and Backes, W.L. (2009) Measurement of Membrane-Bound Human Heme Oxygenase-1 Activity Using a Chemically Defined Assay System, Drug Metab. Dispos. 37, 857-864.

Huber III, W.J., Scruggs, B., and Backes, W.L. (2009) C-Terminal Membrane Spanning Region of Human Heme Oxygenase-1 Mediates a Time Dependent Complex Formation with Cytochrome P450 Reductase, Biochemistry 41, 190-197.

Reed, J.R., Brignac-Huber, L.M., Backes, W.L. (2008) Physical Incorporation of NADPH-cytochrome P450 Reductase and Cytochrome P450 into Phospholipid Vesicles using Glycocholate and Biobeads. Drug Metab. Dispos. 36, 582–588.

Huber, W.J. and Backes, W.L. (2008) Quantitation of Heme Oxygenase-1: Heme Titration Increases Yield of Purified Protein, Anal. Biochem. 373, 167-169.

Cheng, D., Reed, J.R. Harris, D., and Backes, W.L. (2007) Inhibition of CYP2B4 by the Mechanism-based inhibitor 2-Ethynylnaphthalene: Inhibitory Potential of 2EN is Dependent on Substrate Size. Arch. Biochem. Biophys. 462, 28-37.

Cheng, D., Harris, D., Reed, J.R. and Backes, W.L. (2007) Inhibition of CYP2B4 by the Mechanism-based inhibitor 2-Ethynylnaphthalene: Evidence for the Co-Binding of Substrate and Inhibitor within the Active Site. Arch. Biochem. Biophys. 468, 174-182.

Huber, W.J. and Backes, W.L. (2007) Expression and Characterization of Full-Length Human Heme Oxygenase-1: Presence of intact membrane-binding region leads to increased binding affinity for NADPH-cytochrome P450 reductase. Biochemistry 46, 12212-12219.

Kelley, R.W., Cheng, D., and Backes, W.L. (2006) Heteromeric Complex Formation Between CYP2E1 and CYP1A2: Evidence for the Involvement of Electrostatic Interactions. Biochemistry 45, 15807-15816.

Cormier, S.A., Lomnicki, S., Backes, W., and Dellinger, B. (2006) Origin and Health Impacts of Emissions of Toxic By-Products and Fine Particles from Combustion and Thermal Treatment of Hazardous Wastes and Materials. Environmental Health Persp. 114, 1-10.

Reed, J.R., Kelley, R.W., and Backes, W.L.  (2006) An Evaluation of Methods for the Reconstitution of Cytochromes P450 and NADPH P450 Reductase into Lipid Vesicles.  Drug Metab. Dispos. 34, 660-666.

Backes, W.L., Kelley, R.W., Cheng, D., and Reed, J.R. (2005) How are NADPH-cytochrome P450 Reductase and Multiple Cytochromes P450 Organized in Membranes?  Proceedings from the 14th International Conference on Cytochrome P450: Biochemistry, Biophysics and Bioinformatics 163-170.

Kelley, R.W., Reed, J.R. and Backes, W.L. (2005) Effects of Ionic Strength on the Functional Interactions between CYP2B4 and CYP1A2.  Biochemistry, 44, 2632-2641.